Microarray-in-a-tube for detection of multiple viruses.

ABSTRACT:

Background

The detection of multiple viruses is important for pathogenic diagnosis and disease control. Microarray detection is a good method, but requires complex procedures for multiple virus detection.

Methods

We developed a novel PCR assay, the microarray-in-a-tube system, which integrates multiple PCR processes and DNA microarrays for multiple virus detection. A 5 x 5 oligonucleotide microarray for detecting 4 respiratory tract viruses (severe acute respiratory syndrome-associated coronavirus, influenza A virus, influenza B virus, and enterovirus) with inner controls was arranged on the inner surface of a specially designed Eppendorf cap with a flat, optically transparent window.

Results

We were able to perform all detection processes in the encapsulated system w

SUBMITTER: Liu Q 

PROVIDER: S-EPMC7108471 | biostudies-literature | 2007 Feb

REPOSITORIES: biostudies-literature