Complementary Fluorescence Emission and Second Harmonic Spectra Improve Bilayer Characterization.

ABSTRACT: Complementary investigation of Laurdan fluorescence emission and second harmonic (SH) spectra in nonpolar, protic and aprotic polar solvents and phospholipid bilayers was carried out. SH of spectra computed using methods familiar in electro spin resonance spectroscopy yielded better resolution. Spectra were fit to log-normal distributions. SH spectra showed presence of two emissions in protic polar and nonpolar solvents and in both bilayer gel and liquid phases and a single line in aprotic polar solvents. Each of the half maximal positions of each line in both homogenous solvents and bilayers, expresses similar linearity with peak position. This shared feature suggests planar and nonplanar Laurdan conformation respectively in the longer (red) and shorter (blue) wavelength emitting states.

SUBMITTER: Ranganathan R 

PROVIDER: S-EPMC7108755 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature