Simultaneous tracers and a unified model of positional and mass isotopomers for quantification of metabolic flux in liver.

ABSTRACT: Computational models based on the metabolism of stable isotope tracers can yield valuable insight into the metabolic basis of disease. The complexity of these models is limited by the number of tracers and the ability to characterize tracer labeling in downstream metabolites. NMR spectroscopy is ideal for multiple tracer experiments since it precisely detects the position of tracer nuclei in molecules, but it lacks sensitivity for detecting low-concentration metabolites. GC-MS detects stable isotope mass enrichment in low-concentration metabolites, but lacks nuclei and positional specificity. We performed liver perfusions and in vivo infusions of ²H and ¹³C tracers, yielding complex glucose isotopomers that were assigned by NMR and fit to a newly developed metabolic m

SUBMITTER: Deja S 

PROVIDER: S-EPMC7108978 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature