TRAF3IP3 at the trans-Golgi network regulates NKT2 maturation via the MEK/ERK signaling pathway.

ABSTRACT: Thymic natural killer T (NKT)2 cells are a subset of invariant NKT cells with PLZF^hiGATA3^hiIL-4⁺. The differentiation of NKT2 cells is not fully understood. In the present study, we report an important role of TRAF3-interacting protein 3 (TRAF3IP3) in the functional maturation and expansion of committed NKT2s in thymic medulla. Mice with T-cell-specific deletion of TRAF3IP3 had decreased thymic NKT2 cells, decreased IL-4-producing peripheral iNKTs, and defects in response to α-galactosylceramide. Positive selection and high PLZF expression in CD24⁺CD44^- and CCR7⁺CD44^- immature iNKTs were not affected. Only CD44^hiNK1.1^- iNKTs in Traf3ip3^-/- mice showed reduced expression of Egr2, PL

SUBMITTER: Zhang X 

PROVIDER: S-EPMC7109081 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature