Enteral delivery of proteins enhances the expression of proteins involved in the cytoskeleton and protein biosynthesis in human duodenal mucosa.

ABSTRACT:

Background

Amino acids are well known to be key effectors of gut protein turnover. We recently reported that enteral delivery of proteins markedly stimulated global duodenal protein synthesis in carbohydrate-fed healthy humans, but specifically affected proteins remain unknown.

Objective

We aimed to assess the influence of an enteral protein supply on the duodenal mucosal proteome in carbohydrate-fed humans.

Design

Six healthy volunteers received for 5 h, on 2 occasions and in random order, either an enteral infusion of maltodextrins alone (0.25 g · kg⁻¹ · h⁻¹) mimicking the fed state or maltodextrins with a protein powder (0.14 g proteins · kg⁻¹ · h⁻¹). Endoscopic duodenal biopsy specimens were then collected and frozen until analysis. A 2-dimensional polyacrylamide

SUBMITTER: Goichon A 

PROVIDER: S-EPMC7109707 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature