CD4+ T helper cell responses against human bocavirus viral protein 2 viruslike particles in healthy adults.

ABSTRACT:

Background

Human bocavirus (HBoV) was recently described as a new member of the Parvoviridae family, and its possible association with respiratory illness in infants has been discussed. To date, HBoV genomes have been detected worldwide in respiratory tract samples obtained from children with pulmonary diseases, whereas only limited data on virus-specific immunity are available, mainly because of the lack of recombinant viral antigens.

Methods

HBoV viruslike particles (VLPs) were produced in insect cells and characterized by electron microscopy and cesium chloride gradient centrifugation. HBoV viral protein 2 (VP2)-specific antibodies and CD4+ T helper cell responses were analyzed by enzyme-linked immunosorbent assay and enzyme-linked immunospot assay.

Results

VP2 cap

SUBMITTER: Lindner J 

PROVIDER: S-EPMC7109795 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature