Aptamers that bind specifically to human KPNA2 (importin-α1) and efficiently interfere with nuclear transport.

ABSTRACT: The importin-α family of proteins plays an important role in the eukaryotic importin/exportin nuclear transport system. These proteins recognize a nuclear localization signal (NLS) within cargo proteins and import them into the nucleus through nuclear pores, in a process mediated by importin-β. Recent studies have shown that importin-α proteins specifically recognize the NLS of several cellular factors and viral proteins, thus regulating their movement. Dysregulation of importin-α is a common hallmark of many pathologies including, multiple cancers. In this study, we isolated aptamers 76 and 72, which bind specifically and efficiently to KPNA2, a member of a subfamily of importin-α1. Both of these aptamers bind to KPNA2 with an equilibrium dissociation constant (K _d) of 150 nM a

SUBMITTER: Yasuhara N 

PROVIDER: S-EPMC7110145 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature