Identification of differentially expressed circulating exosomal lncRNAs in IgA nephropathy patients.

ABSTRACT:

Background

Although immunoglobulin A nephropathy (IgAN) is one of the foremost primary glomerular disease, treatment of IgAN is still in infancy. Non-invasive biomarkers are urgently needed for IgAN diagnosis. We investigate the difference in expression profiles of exosomal long non-coding-RNAs (lncRNAs) in plasma from IgAN patients compared with their healthy first-degree relatives, which may reveal novel non-invasive IgAN biomarkers.

Methods

We isolated exosomes from the plasma of both IgAN patients and their healthy first-degree relatives. High-throughput RNA sequencing and real-time quantitative polymerase chain reaction (qRT-PCR) was used to validate lncRNA expression profiles. Pathway enrichment analysis was used to predict their nearest protein-coding genes.

Result

SUBMITTER: Guo N 

PROVIDER: S-EPMC7110735 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature