TCF 4 tumor suppressor: a molecular target in the prognosis of sporadic colorectal cancer in humans.

ABSTRACT:

Background

A huge array of function is played by the Wnt/β-catenin signaling pathway in development by balancing gene expression through the modulation of cell-specific DNA binding downstream effectors such as T-cell factor/lymphoid enhancer factor (TCF/LEF). The β-catenin/TCF-4 complex is a central regulatory switch for differentiation and proliferation of intestinal cells (both normal and malignant). Thus, in the present study we evaluated each of 60 cases of sporadic adenocarcinoma, alongside adjoining and normal mucosa specimens of colorectum in humans, for mutation and expression analysis of the gene coding for TCF-4 protein.

Methods

DNA sequencing following PCR amplification and SSCP analysis (single strand conformation polymorphism) was employed to detect TCF-4 gene m

SUBMITTER: Anwar M 

PROVIDER: S-EPMC7110825 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature