Investigation of the immunogenicity of Zika glycan loop.

ABSTRACT:

Background

Zika virus (ZIKV) is a major human pathogen and member of the Flavivirus genus. Previous studies have identified neutralizing antibodies from Zika patients that bind to quaternary epitopes across neighboring envelope (E) proteins, called E dimer epitopes (EDE). An asparagine-linked glycan on the "glycan loop" (GL) of the ZIKV envelope protein protects the functionally important "fusion loop" on the opposite E subunit in the dimer, and EDE antibodies have been shown to bind to both of these loops. Human EDE antibodies have been divided into two subclasses based on how they bind to the glycan loop region: EDE1 antibodies do not require glycosylation for binding, while EDE2 antibodies strongly rely on the glycan for binding.

Methods

ZIKV GL was expressed on tobacco m

SUBMITTER: Henderson EA 

PROVIDER: S-EPMC7110905 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature