Single particle assay of coronavirus membrane fusion with proteinaceous receptor-embedded supported bilayers.

ABSTRACT: Total internal reflection microscopy combined with microfluidics and supported bilayers is a powerful, single particle tracking (SPT) platform for host-pathogen membrane fusion studies. But one major inadequacy of this platform has been capturing the complexity of the cell membrane, including membrane proteins. Because of this, viruses requiring proteinaceous receptors, or other unknown cellular co-factors, have been precluded from study. Here we describe a general method to integrate proteinaceous receptors and cellular components into supported bilayers for SPT fusion studies. This method is general to any enveloped virus-host cell pair, but demonstrated here for feline coronavirus (FCoV). Supported bilayers are formed from mammalian cell membrane vesicles that express feline aminopeptid

SUBMITTER: Costello DA 

PROVIDER: S-EPMC7111216 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature