A novel biosensor based on serum antibody immobilization for rapid detection of viral antigens.

ABSTRACT: In this paper, we represent a label-free biosensor based on immobilization of serum antibodies for rapid detection of viral antigens. Human serum containing specific antibodies against Japanese encephalitis virus (JEV) was immobilized on a silanized surface of an interdigitated sensor via protein A/glutaraldehyde for electrical detection of JEV antigens. The effective immobilization of serum antibodies on the sensor surface was verified by Fourier transform infrared spectrometry and fluorescence microscopy. The signal of the biosensor obtained by the differential voltage converted from the change into non-Faradic impedance resulting from the specific binding of JEV antigens on the surface of the sensor. The detection analyzed indicates that the detection range of this biosensor is 1-10 μg/

SUBMITTER: Huy TQ 

PROVIDER: S-EPMC7111752 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature