Human borna disease virus infection impacts host proteome and histone lysine acetylation in human oligodendroglia cells.

ABSTRACT:

Background

Borna disease virus (BDV) replicates in the nucleus and establishes persistent infections in mammalian hosts. A human BDV strain was used to address the first time, how BDV infection impacts the proteome and histone lysine acetylation (Kac) of human oligodendroglial (OL) cells, thus allowing a better understanding of infection-driven pathophysiology in vitro.

Methods

Proteome and histone lysine acetylation were profiled through stable isotope labeling for cell culture (SILAC)-based quantitative proteomics. The quantifiable proteome was annotated using bioinformatics. Histone acetylation changes were validated by biochemistry assays.

Results

Post BDV infection, 4383 quantifiable differential proteins were identified and functionally annotated to metabolism p

SUBMITTER: Liu X 

PROVIDER: S-EPMC7112117 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature