Nuclear transcriptional changes in hypothalamus of Pomc enhancer knockout mice after excessive alcohol drinking.

ABSTRACT: Persistent alterations of proopiomelanocortin (Pomc) and mu-opioid receptor (Oprm1) activity and stress responses after alcohol are critically involved in vulnerability to alcohol dependency. Gene transcriptional regulation altered by alcohol may play important roles. Mice with genome-wide deletion of neuronal Pomc enhancer1 (nPE1^-/- ), had hypothalamic-specific partial reductions of beta-endorphin and displayed lower alcohol consumption, compared to wildtype littermates (nPE1^+/+ ). We used RNA-Seq to measure steady-state nuclear mRNA transcripts of opioid and stress genes in hypothalamus of nPE1^+/+ and nPE1^-/- mice after 1-day acute withdrawal from chronic excessive alcohol drinking or after water. nPE1^-/- had lower basal Pomc and Pdy

SUBMITTER: Zhou Y 

PROVIDER: S-EPMC7112180 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature