Synthetic virus-like particles prepared via protein corona formation enable effective vaccination in an avian model of coronavirus infection.

ABSTRACT: The ongoing battle against current and rising viral infectious threats has prompted increasing effort in the development of vaccine technology. A major thrust in vaccine research focuses on developing formulations with virus-like features towards enhancing antigen presentation and immune processing. Herein, a facile approach to formulate synthetic virus-like particles (sVLPs) is demonstrated by exploiting the phenomenon of protein corona formation induced by the high-energy surfaces of synthetic nanoparticles. Using an avian coronavirus spike protein as a model antigen, sVLPs were prepared by incubating 100 nm gold nanoparticles in a solution containing an optimized concentration of viral proteins. Following removal of free proteins, antigen-laden particles were recovered and showed morpho

SUBMITTER: Chen HW 

PROVIDER: S-EPMC7112462 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature