Genotype shift in human coronavirus OC43 and emergence of a novel genotype by natural recombination.

ABSTRACT:

Background

Human coronavirus (HCoV) OC43 is the most prevalent HCoV in respiratory tract infections. Its molecular epidemiological characterization, particularly the genotyping, was poorly addressed.

Methods

The full-length spike (S), RNA-dependent RNA polymerase (RdRp), and nucleocapsid (N) genes were amplified from each respiratory sample collected from 65 HCoV-OC43-positive patients between 2005 and 2012. Genotypes were determined by phylogenetic analysis. Recombination was analyzed based on full-length viral genome sequences. Clinical manifestations of each HCoV genotype infection were compared by reviewing clinical records.

Results

Sixty of these 65 samples belong to genotypes B, C and D. The remaining five strains had incongruent positions in the phylogenetic tr

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC7112537 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature