Identifcation of differentially expressed long non-coding RNAs in CD4+ T cells response to latent tuberculosis infection.

ABSTRACT:

Objective

To identify differentially expressed long non-coding RNAs (lncRNAs) in CD4(+) T cells triggered upon latent tuberculosis (TB) infection.

Methods

Expression profiles of lncRNAs and mRNAs in CD4(+) T cells from individuals with latent TB infection (LTBI), active TB and healthy controls were analyzed by microarray assay and four lncRNAs were selected for validation using real time-quantitative polymerase chain reaction (RT-qPCR). Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway based approaches were used to investigate biological functions and signaling pathways affected by the differentially expressed mRNAs.

Results

LncRNAs and mRNAs in CD4(+) T cells were involved in LTBI and active TB disease. Compared with healthy controls, 449

SUBMITTER: Yi Z 

PROVIDER: S-EPMC7112653 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature