Multiparameter Analysis Identifies Heterogeneity in Knee Osteoarthritis Synovial Responses.

ABSTRACT:

Objective

Synovial membrane inflammation is common in osteoarthritis (OA) and increases cartilage injury. However, synovial fluid and histology studies suggest that OA inflammatory responses are not homogeneous. Greater understanding of these responses may provide new insights into OA disease mechanisms. We undertook this study to develop a novel multiparameter approach to phenotype synovial responses in knee OA.

Methods

Cell composition and soluble protein production were measured by flow cytometry and multiplex enzyme-linked immunosorbent assay in synovium collected from OA patients undergoing knee replacement surgery (n = 35).

Results

Testing disaggregation conditions showed that aggressive digestion improved synovial cell yield and mesenchymal staining by flow cyt

SUBMITTER: Labinsky H 

PROVIDER: S-EPMC7113107 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature