An open-source robotic platform that enables automated monitoring of replicate biofilm cultivations using optical coherence tomography.

ABSTRACT: The paper introduces a fully automated cultivation and monitoring tool to study biofilm development in replicate experiments operated in parallel. To gain a fundamental understanding of the relation between cultivation conditions and biofilm characteristics (e.g., structural, mechanical) a monitoring setup allowing for the standardization of methods is required. Optical coherence tomography (OCT) is an imaging modality ideal for biofilms since it allows for the monitoring of structure in real time. By integrating an OCT device into the open-source robotic platform EvoBot, a fully automated monitoring platform for investigating biofilm development in several flow cells at once was realized. Different positioning scenarios were tested and revealed that the positioning accuracy is within the

SUBMITTER: Gierl L 

PROVIDER: S-EPMC7113294 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature