Induction of Synthetic Polyploids and Assessment of Genomic Stability in Lippia alba.

ABSTRACT: Polyploidy is widely recognized as a major evolutionary force in plants and has been reported in the genus Lippia (Verbenaceae). Lippia alba, the most studied species, has been documented as a polyploid complex involving at least four ploidal levels. L. alba presents remarkable chemical and genetic variation and represents a model for understanding genome organization. Although the economic and medicinal importance of the species has been widely described, no established polyploid induction protocol has been reported so far. Here, we describe the production of synthetic polyploid plants of L. alba using colchicine. The ploidal levels were estimated by flow cytometry and chromosome counting. In addition, FISH and molecular markers approaches were used to confirm

SUBMITTER: Juliao SA 

PROVIDER: S-EPMC7113378 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature