P300 and cAMP response element-binding protein-binding protein in skeletal muscle homeostasis, contractile function, and survival.

ABSTRACT:

Background

Reversible ε-amino acetylation of lysine residues regulates transcription as well as metabolic flux; however, roles for specific lysine acetyltransferases in skeletal muscle physiology and function are unknown. In this study, we investigated the role of the related acetyltransferases p300 and cAMP response element-binding protein-binding protein (CBP) in skeletal muscle transcriptional homeostasis and physiology in adult mice.

Methods

Mice with skeletal muscle-specific and inducible knockout of p300 and CBP (PCKO) were generated by crossing mice with a tamoxifen-inducible Cre recombinase expressed under the human α-skeletal actin promoter with mice having LoxP sites flanking exon 9 of the Ep300 and Crebbp genes. Knockout of PCKO was induced at 13-15 weeks of age v

SUBMITTER: Svensson K 

PROVIDER: S-EPMC7113519 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature