Structure, dynamics and immunogenicity of a catalytically inactive C<i>X</i>C chemokine-degrading protease SpyCEP from <i>Streptococcus pyogenes</i>.

ABSTRACT: Over 18 million disease cases and half a million deaths worldwide are estimated to be caused annually by Group A Streptococcus. A vaccine to prevent GAS disease is urgently needed. SpyCEP (Streptococcus pyogenes Cell-Envelope Proteinase) is a surface-exposed serine protease that inactivates chemokines, impairing neutrophil recruitment and bacterial clearance, and has shown promising immunogenicity in preclinical models. Although SpyCEP structure has been partially characterized, a more complete and higher resolution understanding of its antigenic features would be desirable prior to large scale manufacturing. To address these gaps and facilitate development of this globally important vaccine, we performed immunogenicity studies with a safety-engineered SpyCEP mutant, and comprehensi

SUBMITTER: McKenna S 

PROVIDER: S-EPMC7113628 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature