Plant Chromosome-Specific Probes by Microdissection of a Single Chromosome: Is That a Reality?

ABSTRACT: Painting plant chromosomes through chromosomal in situ suppression (CISS) hybridization has long been considered impracticable. Seeking to build specific and complex probes from a single microdissected chromosome, we employed human chromosomes as models to standardize all the necessary steps for application in plants. Human metaphases were used to define the adequate conditions for microdissection, chromosome DNA amplification and labeling through degenerate oligonucleotide-primed PCR, and in situ hybridization stringency. Subsequently, these methodologies were applied in the plant species Zea mays (chromosome 1) and Capsicum annuum (chromosome 7 or 8). The high quality of human and plant cytogenetic preparations and the meticulous standardization of each step,

SUBMITTER: Soares FAF 

PROVIDER: S-EPMC7113637 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature