A novel real-time PCR assay panel for detection of common respiratory pathogens in a convenient, strip-tube array format.

ABSTRACT: Commercial multiplex assays, built on different chemistries and platforms are widely available for simultaneous detection of pathogens that cause respiratory infections. However, these tests are often difficult to implement in a resource limited setting because of high cost. In this study, we developed and validated a method for simultaneous testing of common respiratory pathogens (Respanel) by real-time PCR in a convenient, strip-tube array format. Primers and probes for sixteen PCR assays were selected from the literature or newly designed. Following optimization of individual PCR assays, strip-tube arrays were prepared by dispensing primer-probe mixes (PPM) into two sets of 8-tube strips. Nucleic acid extracts from specimens were mixed with PCR master mix, and dispensed column-wise into

SUBMITTER: Hasan MR 

PROVIDER: S-EPMC7113679 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature