A method for the improved detection of aerosolized influenza viruses and the male-specific (F+) RNA coliphage MS2.

ABSTRACT: The detection of aerosolized viruses can serve as an important surveillance and control tool in agriculture, human health, and environmental settings. Here, we adapted an anion exchange resin-based method, initially developed to concentrate negatively charged viruses from water, to liquid impingement-based bioaerosol sampling. In this method, aerosolized viruses are collected in a 20ml liquid sample contained within widely used impingers, BioSamplers (SKC Inc., Eighty Four, PA), and further concentrated via adsorption to an anion exchange resin that is suspended within this liquid. Viral nucleic acids are then extracted from the resin to facilitate molecular analyses through a reduction in the effective sample volume. For this study, various quantities of two negatively charged viruses, ty

SUBMITTER: Chandler JC 

PROVIDER: S-EPMC7113747 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature