Development and Evaluation of a One-Step Quantitative RT-PCR Assay for Detection of Lassa Virus.

ABSTRACT: Lassa fever is a severe viral hemorrhagic illness caused by Lassa virus. Based on estimates, the number of LASV infections ranges from 300,000 to 500,000 cases in endemic areas with a fatality rate of 1%. Development of fast and sensitive tools for the control and prevention of Lassa virus infection as well as for clinical diagnostics of Lassa fever are crucial. Here we reported development and evaluation of a one-step quantitative RT-qPCR assay for the Lassa virus detection - LASV-Fl. This assay is suitable for the detection of lineages I-IV of Lassa virus. The limit of detection of the assay ranged from 10³ copies/ml to 10⁵ copies/ml and has 96.4% diagnostic sensitivity, whereas analytical and diagnostic specificities both were 100%. Serum, whole blood and tissue ar

SUBMITTER: Dedkov VG 

PROVIDER: S-EPMC7113850 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature