Rapid one-step construction of a Middle East Respiratory Syndrome (MERS-CoV) infectious clone system by homologous recombination.

ABSTRACT:

Background

Viral Infectious clone systems serve as robust platforms to study viral gene or replicative function by reverse genetics, formulate vaccines and adapt a wild type-virus to an animal host. Since the development of the first viral infectious clone system for the poliovirus, novel strategies of viral genome construction have allowed for the assembly of viral genomes across the identified viral families. However, the molecular profiles of some viruses make their genome more difficult to construct than others. Two factors that affect the difficulty of infectious clone construction are genome length and genome complexity.

Results

This work examines the available strategies for overcoming the obstacles of assembling the long and complex RNA genomes of coronaviruses and r

SUBMITTER: Nikiforuk AM 

PROVIDER: S-EPMC7113859 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature