Development of a novel DNA-launched dengue virus type 2 infectious clone assembled in a bacterial artificial chromosome.

ABSTRACT: Major progress in Dengue virus (DENV) biology has resulted from the use of infectious clones obtained through reverse genetics. The construction of these clones is commonly based on high- or low-copy number plasmids, yeast artificial chromosomes, yeast-Escherichia coli shuttle vectors, and bacterial artificial chromosomes (BACs). Prokaryotic promoters have consistently been used for the transcription of these clones. The goal of this study was to develop a novel DENV infectious clone in a BAC under the control of the cytomegalovirus immediate-early promoter and to generate a virus with the fusion envelope-green fluorescent protein in an attempt to track virus infection. The transfection of Vero cells with a plasmid encoding the DENV infectious clone facilitated the recovery of infectious p

SUBMITTER: Usme-Ciro JA 

PROVIDER: S-EPMC7114509 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature