Vitamin B₁₂ is neuroprotective in experimental pneumococcal meningitis through modulation of hippocampal DNA methylation.

ABSTRACT:

Background

Bacterial meningitis (BM) causes apoptotic damage to the hippocampus and homocysteine (Hcy) accumulation to neurotoxic levels in the cerebrospinal fluid of children. The Hcy pathway controls bioavailability of methyl, and its homeostasis can be modulated by vitamin B₁₂, a cofactor of the methionine synthase enzyme. Herein, the neuroprotective potential and the underlying mode of action of vitamin B₁₂ adjuvant therapy were assessed in an infant rat model of BM.

Methods

Eleven-day old rats were intracysternally infected with Streptococcus pneumoniae serotype 3, or saline, treated with B₁₂ or placebo, and, 24 h after infection, their hippocampi were analyzed for apoptosis in the dentate gyrus, sulfur amino acids content, global DNA me

SUBMITTER: de Queiroz KB 

PROVIDER: S-EPMC7115084 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature