Interplay between RNA-binding protein HuR and Nox4 as a novel therapeutic target in diabetic kidney disease.

ABSTRACT:

Objective

Glomerular injury is a prominent pathological feature of diabetic kidney disease (DKD). Constitutively active NADPH oxidase 4 (Nox4) is a major source of reactive oxygen species that mediates hyperglycemia-induced mesangial cell (MC) fibrotic injury. However, the mechanism that Nox4 utilizes to achieve its biological outcome remains elusive, and the signaling pathways that regulate this isoform oxidase are not well understood. Here, our goal is to study the detailed mechanism by which NAPDH oxidase 4 (Nox4) is post-transcriptionally regulated in MC during diabetic pathology.

Methods

We studied the protein expression of HuR, Nox4 and matrix proteins by western blotting, while we assessed the mRNA stability of Nox4 by RT-PCR and polysomal assay, examined in vitro cul

SUBMITTER: Shi Q 

PROVIDER: S-EPMC7115155 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature