Differential effects of a common splice site polymorphism on the generation of OAS1 variants in human bronchial epithelial cells.

ABSTRACT: The 2',5'-oligoadenylate synthetase 1 (OAS1) is one of the major interferon-inducible proteins and a critical component of the host defense system against viral infection. A single nucleotide polymorphism (SNP), rs10774671, presumably responsible for alternate splicing of this gene, has frequently been associated with a variety of viral diseases, including emerging respiratory infections. We investigated the SNP-dependent expression of OAS1 variants in primary cultured human bronchial epithelial cells. Total RNA was subjected to real-time RT-PCR with specific primer sets designed to amplify each transcript variant. We found that the p46 transcript was mainly expressed in cells with the GG genotype, whereas the p42 transcript was highly expressed, and the p44a (alternate exon in intron 5),

SUBMITTER: Noguchi S 

PROVIDER: S-EPMC7115495 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature