Characterizing metabolic stress-induced phenotypes of Synechocystis PCC6803 with Raman spectroscopy.

ABSTRACT:

Background

During their long evolution, Synechocystis sp. PCC6803 developed a remarkable capacity to acclimate to diverse environmental conditions. In this study, Raman spectroscopy and Raman chemometrics tools (Rametrix^TM) were employed to investigate the phenotypic changes in response to external stressors and correlate specific Raman bands with their corresponding biomolecules determined with widely used analytical methods.

Methods

Synechocystis cells were grown in the presence of (i) acetate (7.5-30 mM), (ii) NaCl (50-150 mM) and (iii) limiting levels of MgSO₄ (0-62.5 mM) in BG-11 media. Principal component analysis (PCA) and discriminant analysis of PCs (DAPC) were performed with the Rametrix^TM LITE Toolbox for MATLAB^Ⓡ

SUBMITTER: Tanniche I 

PROVIDER: S-EPMC7115747 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature