Bipartite interactions, antibiotic production and biosynthetic potential of the Arabidopsis leaf microbiome.

ABSTRACT: Plants are colonized by phylogenetically diverse microorganisms that affect plant growth and health. Representative genome-sequenced culture collections of bacterial isolates from model plants, including Arabidopsis thaliana, have recently been established. These resources provide opportunities for systematic interaction screens combined with genome mining to discover uncharacterized natural products. Here, we report on the biosynthetic potential of 224 strains isolated from the A. thaliana phyllosphere. Genome mining identified more than 1,000 predicted natural product biosynthetic gene clusters (BGCs), hundreds of which are unknown compared to the MIBiG database of characterized BGCs. For functional validation, we used a high-throughput screening approach to monitor over 50,000 binary st

SUBMITTER: Helfrich EJN 

PROVIDER: S-EPMC7115891 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature