Alternate splicing of transcripts upon Mycobacterium tuberculosis infection impacts the expression of functional protein domains.

ABSTRACT: Previously, we reported that infection of human macrophages with Mycobacterium tuberculosis (Mtb) results in massive alterations in the pattern of RNA splicing in the host. The finding gained significance since alternate spliced variants of a same gene may have substantially different structure, function, stability, interaction partners, localization, and so forth, owing to inclusion or exclusion of specific exons. To establish a proof-of-concept; on how infection-induced RNA splicing could impact protein functions, here we used RNA-seq data from THP-1 macrophages that were infected with clinical isolate of Mtb. In addition to re-establishing the fact that Mtb infection may cause strain specific alterations in RNA splicing, we also developed a new analysis pipeline resulting in characteriz

SUBMITTER: Kalam H 

PROVIDER: S-EPMC7115969 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature