Retapamulin-Assisted Ribosome Profiling Reveals the Alternative Bacterial Proteome.

ABSTRACT: The use of alternative translation initiation sites enables production of more than one protein from a single gene, thereby expanding the cellular proteome. Although several such examples have been serendipitously found in bacteria, genome-wide mapping of alternative translation start sites has been unattainable. We found that the antibiotic retapamulin specifically arrests initiating ribosomes at start codons of the genes. Retapamulin-enhanced Ribo-seq analysis (Ribo-RET) not only allowed mapping of conventional initiation sites at the beginning of the genes, but strikingly, it also revealed putative internal start sites in a number of Escherichia coli genes. Experiments demonstrated that the internal start codons can be recognized by the ribosomes and direct translation initiation in vit

SUBMITTER: Meydan S 

PROVIDER: S-EPMC7115971 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature