IgA-producing plasma cells originate from germinal centers that are induced by B-cell receptor engagement in humans.

ABSTRACT:

Background & aims

IgA contributes to homeostatic balance between host and intestinal microbiota. Mechanisms that initiate the IgA response are unclear and likely to differ between humans and animal models. We used multiple experimental approaches to investigate the origin of human intestinal plasma cells that produce IgA in the gastrointestinal tract.

Methods

Complexity of IgA-producing plasma cell populations in human gastrointestinal mucosa and bone marrow and the specific response to oral cholera vaccine were compared by analysis of immunoglobulin genes. Flow cytometry, gene expression analysis, and immunohistochemistry were used to analyze signaling pathways induced by B-cell receptor engagement in human gut-associated lymphoid tissue (GALT) and involvement of innate imm

SUBMITTER: Barone F 

PROVIDER: S-EPMC7115992 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature