Long-term live-cell microscopy with labeled nanobodies delivered by laser-induced photoporation.

ABSTRACT: Fluorescence microscopy is the method of choice for studying intracellular dynamics. However, its success depends on the availability of specific and stable markers. A prominent example of markers that are rapidly gaining interest are nanobodies (Nbs, ~ 15 kDa), which can be functionalized with bright and photostable organic fluorophores. Due to their relatively small size and high specificity, Nbs offer great potential for high-quality long-term subcellular imaging, but suffer from the fact that they cannot spontaneously cross the plasma membrane of live cells. We have recently discovered that laser-induced photoporation is well suited to deliver extrinsic labels to living cells without compromising their viability. Being a laser-based technology, it is readily compatible with light micro

SUBMITTER: Liu J 

PROVIDER: S-EPMC7116313 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature