Comparative evaluation of DNA-encoded chemical selections performed using DNA in single-stranded or double-stranded format.

ABSTRACT: DNA-encoded chemical libraries (DEL) are increasingly being used for the discovery and optimization of small organic ligands to proteins of biological or pharmaceutical interest. The DNA fragments, that serve as amplifiable identification barcodes for individual compounds in the library, are typically used in double-stranded DNA format. To the best of our knowledge, a direct comparison of DEL selections featuring DNA in either single- or double-stranded DNA format has not yet been reported. In this article, we describe a comparative evaluation of selections with two DEL libraries (named GB-DEL and NF-DEL), based on different chemical designs and produced in both single- and double-stranded DNA format. The libraries were selected in identical conditions against multiple protein targets, rev

SUBMITTER: Bassi G 

PROVIDER: S-EPMC7116422 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature