Engineering transplantable jejunal mucosal grafts using patient-derived organoids from children with intestinal failure.

ABSTRACT: Intestinal failure, following extensive anatomical or functional loss of small intestine, has debilitating long-term consequences for children¹. The priority of patient care is to increase the length of functional intestine, particularly the jejunum, to promote nutritional independence². Here we construct autologous jejunal mucosal grafts using biomaterials from pediatric patients and show that patient-derived organoids can be expanded efficiently in vitro. In parallel, we generate decellularized human intestinal matrix with intact nanotopography, which forms biological scaffolds. Proteomic and Raman spectroscopy analyses reveal highly analogous biochemical profiles of human small intestine and colon scaffolds, indicating that they can be used interchangeably as platf

SUBMITTER: Meran L 

PROVIDER: S-EPMC7116539 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature