Evaluating High-Throughput Sequencing Data of Microalgae Living in Melting Snow: Improvements and Limitations¹.

ABSTRACT: Melting snow fields are an extremophilic habitat dominated by closely related Chlamydomonadaceae (Chlorophyta). Microscopy-based classification of these cryophilic microalgae is challenging and may not reveal the true diversity. High-throughput sequencing (HTS) allows for a more comprehensive evaluation of the community. However, HTS approaches have been rarely used in such ecosystems and the output of their application has not been evaluated. Furthermore, there is no consensus on the choice for a suitable DNA marker or data processing workflow. We found that the correct placement of taxonomic strings onto OTUs strongly depends on the quality of the reference databases. We improved the assignments of the HST data by generating additional reference sequences of the locally abundant taxa, gu

SUBMITTER: Lutz S 

PROVIDER: S-EPMC7116558 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature