Conformation of sister chromatids in the replicated human genome.

ABSTRACT: The three-dimensional organization of the genome supports regulated gene expression, recombination, DNA repair, and chromosome segregation during mitosis. Chromosome conformation capture (Hi-C)^1,2 analysis has revealed a complex genomic landscape of internal chromosomal structures in vertebrate cells^3-7, but the identical sequence of sister chromatids has made it difficult to determine how they topologically interact in replicated chromosomes. Here we describe sister-chromatid-sensitive Hi-C (scsHi-C), which is based on labelling of nascent DNA with 4-thio-thymidine and nucleoside conversion chemistry. Genome-wide conformation maps of human chromosomes reveal that sister-chromatid pairs interact most frequently at the boundaries of topologically associating domains (T

SUBMITTER: Mitter M 

PROVIDER: S-EPMC7116725 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature