Replacement of surgical vasectomy through the use of wild-type sterile hybrids.

ABSTRACT: For the production and rederivation of mouse strains, pseudopregnant female mice are used for embryo transfer and serve as surrogate mothers to support embryo development to term. Vasectomized males are commonly used to render pseudopregnancy in females, generated by surgical procedures associated with considerable pain and discomfort. Genetically modified mouse strains with a sterility phenotype provide a non-surgical replacement and represent an important application of the 3Rs (Replacement, Reduction, Refinement). However, the maintenance of such genetically modified mouse strains requires extensive breeding and genotyping procedures, which are regulated procedures under national legislation. As an alternative, we have explored the use of sterile male hybrids that result when two wild-t

SUBMITTER: Preece C 

PROVIDER: S-EPMC7116729 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature