Efficient synthesis of enantiopure amines from alcohols using resting E. coli cells and ammonia.

ABSTRACT: α-Chiral amines are pivotal building blocks for chemical manufacturing. Stereoselective amination of alcohols is receiving increased interest due to its higher atom-efficiency and overall improved environmental footprint compared with other chemocatalytic and biocatalytic methods. We previously developed a hydrogen-borrowing amination by combining an alcohol dehydrogenase (ADH) with an amine dehydrogenase (AmDH) in vitro. Herein, we implemented the ADH-AmDH bioamination in resting Escherichia coli cells for the first time. Different genetic constructs were created and tested in order to obtain balanced expression levels of the dehydrogenase enzymes in E. coli. Using the optimized constructs, the influence of several parameters towards the productivity of the sys

SUBMITTER: Houwman JA 

PROVIDER: S-EPMC7116806 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature