Loop extrusion as a mechanism for formation of DNA damage repair foci.

ABSTRACT: The repair of DNA double-strand breaks (DSBs) is essential for safeguarding genome integrity. When a DSB forms, the PI3K-related ATM kinase rapidly triggers the establishment of megabase-sized, chromatin domains decorated with phosphorylated histone H2AX (γH2AX), which act as seeds for the formation of DNA-damage response foci¹. It is unclear how these foci are rapidly assembled to establish a 'repair-prone' environment within the nucleus. Topologically associating domains are a key feature of 3D genome organization that compartmentalize transcription and replication, but little is known about their contribution to DNA repair processes^2,3. Here we show that topologically associating domains are functional units of the DNA damage response, and are instrumental for the

SUBMITTER: Arnould C 

PROVIDER: S-EPMC7116834 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature