Wapl repression by Pax5 promotes V gene recombination by Igh loop extrusion.

ABSTRACT: Nuclear processes, such as V(D)J recombination, are orchestrated by the three-dimensional organization of chromosomes at multiple levels, including compartments¹ and topologically associated domains (TADs)^2,3 consisting of chromatin loops⁴. TADs are formed by chromatin-loop extrusion^5-7, which depends on the loop-extrusion function of the ring-shaped cohesin complex^8-12. Conversely, the cohesin-release factor Wapl^13,14 restricts loop extension^10,15. The generation of a diverse antibody repertoire, providing humoral immunity to pathogens, requires the participation of all V genes in V(D)J recombination¹⁶, which depends on contraction of the 2.8-Mb-long immunoglobulin heavy chain (Igh) locus by Pax5¹

SUBMITTER: Hill L 

PROVIDER: S-EPMC7116900 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature