A novel intracellularly expressed NS5B-specific nanobody suppresses bovine viral diarrhea virus replication.

ABSTRACT: Bovine viral diarrhea virus (BVDV) infection causes significant economic losses to the cattle industry worldwide and still represents a huge pressure on agricultural production. Thus, the development of novel anti-BVDV strategies are urgently needed. The nonstructural protein 5 (NS5B) of BVDV is essential for viral replication. Further, the camel single-domain antibody (nanobody) represents a promising antiviral approach with the advantages of small size, stable structure, high specificity and solubility, and the recognition of specific epitopes. However, no NS5B-specific nanobodies against BVDV have been reported. In this study, NS5B-specific nanobodies were isolated from a phage display library of variable domains of Camellidae heavy chain-only antibodies (VHHs). Further, an MDBK cell li

SUBMITTER: Duan H 

PROVIDER: S-EPMC7117317 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature