Evidence of defined temporal expression patterns that lead a gram-negative cell out of dormancy.

ABSTRACT: Many bacterial species are capable of forming long-lived dormant cells. The best characterized are heat and desiccation resistant spores produced by many Gram-positive species. Less characterized are dormant cysts produced by several Gram-negative species that are somewhat tolerant to increased temperature and very resistant to desiccation. While there is progress in understanding regulatory circuits that control spore germination, there is scarce information on how Gram-negative organisms emerges from dormancy. In this study, we show that R. centenum cysts germinate by emerging a pair of motile vegetative cells from a thick cyst cell wall coat ~ 6 hrs post induction of germination. Time-lapse transcriptomic analysis reveals that there is a defined temporal pattern of gene expression chang

SUBMITTER: Ashok N 

PROVIDER: S-EPMC7117780 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature