Rapid Identification of Candida Species in Candidemia Directly from Blood Samples Using Imperfect Match Probes.

ABSTRACT: Candidemia is associated with a high mortality rate, and initial adequate antifungal therapy results in a significant decrease in the crude mortality. We herein report a rapid method that can identify eight Candida species in candidemia using imperfect match quenching probes (IM Q-probes) within three and a half hours of whole blood sample collection. Furthermore, employing the D value, which reflects the difference between the Tm signature from a clinical isolate and that registered in the database, it is possible to quickly identify samples suitable for IM Q-probe identification. We first evaluated the method using 34 Candida colonies collected from different patients, and 100% (34/34) of the identification results matched the preidentified Candida species. We then performed blind tests

SUBMITTER: Higashi Y 

PROVIDER: S-EPMC7118160 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature