Continuous chromosome-scale haplotypes assembled from a single interspecies F1 hybrid of yak and cattle.

ABSTRACT:

Background

The development of trio binning as an approach for assembling diploid genomes has enabled the creation of fully haplotype-resolved reference genomes. Unlike other methods of assembly for diploid genomes, this approach is enhanced, rather than hindered, by the heterozygosity of the individual sequenced. To maximize heterozygosity and simultaneously assemble reference genomes for 2 species, we applied trio binning to an interspecies F1 hybrid of yak (Bos grunniens) and cattle (Bos taurus), 2 species that diverged nearly 5 million years ago. The genomes of both of these species are composed of acrocentric autosomes.

Results

We produced the most continuous haplotype-resolved assemblies for a diploid animal yet reported. Both the maternal (yak) and paternal (cattle) as

SUBMITTER: Rice ES 

PROVIDER: S-EPMC7118895 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature