Dried blood spot versus venous blood sampling for phenylalanine and tyrosine.

ABSTRACT:

Background

This study investigated the agreement between various dried blood spot (DBS) and venous blood sample measurements of phenylalanine and tyrosine concentrations in Phenylketonuria (PKU) and Tyrosinemia type 1 (TT1) patients.

Study design

Phenylalanine and tyrosine concentrations were studied in 45 PKU/TT1 patients in plasma from venous blood in lithium heparin (LH) and EDTA tubes; venous blood from LH and EDTA tubes on a DBS card; venous blood directly on a DBS card; and capillary blood on a DBS card. Plasma was analyzed with an amino acid analyzer and DBS were analyzed with liquid chromatography-mass spectrometry. Agreement between different methods was assessed using Passing and Bablok fit and Bland Altman analyses.

Results

In general, phenylalanine concent

SUBMITTER: van Vliet K 

PROVIDER: S-EPMC7118958 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature